Enzyme classification using complex dynamic hemithioacetal systems† †Electronic supplementary information (ESI) available: NMR spectra, linear discriminant analysis. See DOI: 10.1039/c6cc01823h Click here for additional data file.

Reagents were obtained from commercial suppliers and used as received. Lipase preparation PSIm was purchased from Amano Enzyme Inc. The other lipases were purchased from Sigma-Aldrich. 1H-NMR and 13C-NMR data were recorded on a Bruker Avance 400 (100) MHz and/or a Bruker Avance 500 (125) MHz, respectively. Chemical shifts are reported as δ values (ppm) with CDCl3 (1H-NMR δ 7.26, 13C-NMR δ 77.16) as an internal standard. J values are given in Hertz (Hz). High resolution mass spectroscopy was performed by the Instrument Station of National Center for Genetic Engineering and Biotechnology (BIOTEC), Thailand. Thin layer chromatography (TLC) was performed on precoated Polygram® SIL G/UV 254 silica plates (0.20 mm, Macherey-Nagel), visualized with UV-detection. Flash column chromatography was performed on silica gel 60, 0.040-0.063 mm (SDS) or neutral aluminum oxide.